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myelin proteolipid protein plp staining  (Bio-Rad)
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Neuropathological findings across study subjects. (A) Staining with AT8 (Tau) across superior and middle temporal samples from each study subject (left panel). (B) Higher magnification AT8 staining (right panel) from representative grey and white matter regions, indicated by squares in the left panel. (C) Immunohistochemical staining with TDP-43 across the preparations from all study subjects (left panel). (D) Corresponding magnification of grey and white matter regions (right panel), indicated by squares in the left panel. (E) Additional magnification and morphological features of TDP-43 aggregates in GM regions from FTLD subjects. Additional whole-section and high magnification images of grey and white matter stained for β-amyloid (F) and <t>proteolipid</t> protein <t>(PLP)</t> (G) are presented for each frontotemporal lobar dementia case. FTLD, frontotemporal lobar dementia; H-AD, high likelihood Alzheimer’s disease; AT8, phosphorylated tau protein; He, hematoxylin; GM, grey matter; WM, white matter; TDP-43, TAR DNA-binding protein 43; PLP, proteolipid protein. NIIs, intranuclear inclusion; NCIs, neuronal cytoplasmic inclusions; DNs, dystrophic neurites.
Myelin Proteolipid Protein Plp Staining, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paraformaldehyde lysine phosphate plp buffer  (Thermo Fisher)
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Neuropathological findings across study subjects. (A) Staining with AT8 (Tau) across superior and middle temporal samples from each study subject (left panel). (B) Higher magnification AT8 staining (right panel) from representative grey and white matter regions, indicated by squares in the left panel. (C) Immunohistochemical staining with TDP-43 across the preparations from all study subjects (left panel). (D) Corresponding magnification of grey and white matter regions (right panel), indicated by squares in the left panel. (E) Additional magnification and morphological features of TDP-43 aggregates in GM regions from FTLD subjects. Additional whole-section and high magnification images of grey and white matter stained for β-amyloid (F) and <t>proteolipid</t> protein <t>(PLP)</t> (G) are presented for each frontotemporal lobar dementia case. FTLD, frontotemporal lobar dementia; H-AD, high likelihood Alzheimer’s disease; AT8, phosphorylated tau protein; He, hematoxylin; GM, grey matter; WM, white matter; TDP-43, TAR DNA-binding protein 43; PLP, proteolipid protein. NIIs, intranuclear inclusion; NCIs, neuronal cytoplasmic inclusions; DNs, dystrophic neurites.
Paraformaldehyde Lysine Phosphate Plp Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Neuropathological findings across study subjects. (A) Staining with AT8 (Tau) across superior and middle temporal samples from each study subject (left panel). (B) Higher magnification AT8 staining (right panel) from representative grey and white matter regions, indicated by squares in the left panel. (C) Immunohistochemical staining with TDP-43 across the preparations from all study subjects (left panel). (D) Corresponding magnification of grey and white matter regions (right panel), indicated by squares in the left panel. (E) Additional magnification and morphological features of TDP-43 aggregates in GM regions from FTLD subjects. Additional whole-section and high magnification images of grey and white matter stained for β-amyloid (F) and <t>proteolipid</t> protein <t>(PLP)</t> (G) are presented for each frontotemporal lobar dementia case. FTLD, frontotemporal lobar dementia; H-AD, high likelihood Alzheimer’s disease; AT8, phosphorylated tau protein; He, hematoxylin; GM, grey matter; WM, white matter; TDP-43, TAR DNA-binding protein 43; PLP, proteolipid protein. NIIs, intranuclear inclusion; NCIs, neuronal cytoplasmic inclusions; DNs, dystrophic neurites.
Probes Targeting Plp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plp-tta transgenic mice  (BioResource International Inc)
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Neuropathological findings across study subjects. (A) Staining with AT8 (Tau) across superior and middle temporal samples from each study subject (left panel). (B) Higher magnification AT8 staining (right panel) from representative grey and white matter regions, indicated by squares in the left panel. (C) Immunohistochemical staining with TDP-43 across the preparations from all study subjects (left panel). (D) Corresponding magnification of grey and white matter regions (right panel), indicated by squares in the left panel. (E) Additional magnification and morphological features of TDP-43 aggregates in GM regions from FTLD subjects. Additional whole-section and high magnification images of grey and white matter stained for β-amyloid (F) and <t>proteolipid</t> protein <t>(PLP)</t> (G) are presented for each frontotemporal lobar dementia case. FTLD, frontotemporal lobar dementia; H-AD, high likelihood Alzheimer’s disease; AT8, phosphorylated tau protein; He, hematoxylin; GM, grey matter; WM, white matter; TDP-43, TAR DNA-binding protein 43; PLP, proteolipid protein. NIIs, intranuclear inclusion; NCIs, neuronal cytoplasmic inclusions; DNs, dystrophic neurites.
Plp Tta Transgenic Mice, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti plp antibodies  (Bio-Rad)
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Neuropathological findings across study subjects. (A) Staining with AT8 (Tau) across superior and middle temporal samples from each study subject (left panel). (B) Higher magnification AT8 staining (right panel) from representative grey and white matter regions, indicated by squares in the left panel. (C) Immunohistochemical staining with TDP-43 across the preparations from all study subjects (left panel). (D) Corresponding magnification of grey and white matter regions (right panel), indicated by squares in the left panel. (E) Additional magnification and morphological features of TDP-43 aggregates in GM regions from FTLD subjects. Additional whole-section and high magnification images of grey and white matter stained for β-amyloid (F) and <t>proteolipid</t> protein <t>(PLP)</t> (G) are presented for each frontotemporal lobar dementia case. FTLD, frontotemporal lobar dementia; H-AD, high likelihood Alzheimer’s disease; AT8, phosphorylated tau protein; He, hematoxylin; GM, grey matter; WM, white matter; TDP-43, TAR DNA-binding protein 43; PLP, proteolipid protein. NIIs, intranuclear inclusion; NCIs, neuronal cytoplasmic inclusions; DNs, dystrophic neurites.
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plp 139-151 (naive)/cfa emulsion  (Hooke Laboratories)
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Neuropathological findings across study subjects. (A) Staining with AT8 (Tau) across superior and middle temporal samples from each study subject (left panel). (B) Higher magnification AT8 staining (right panel) from representative grey and white matter regions, indicated by squares in the left panel. (C) Immunohistochemical staining with TDP-43 across the preparations from all study subjects (left panel). (D) Corresponding magnification of grey and white matter regions (right panel), indicated by squares in the left panel. (E) Additional magnification and morphological features of TDP-43 aggregates in GM regions from FTLD subjects. Additional whole-section and high magnification images of grey and white matter stained for β-amyloid (F) and <t>proteolipid</t> protein <t>(PLP)</t> (G) are presented for each frontotemporal lobar dementia case. FTLD, frontotemporal lobar dementia; H-AD, high likelihood Alzheimer’s disease; AT8, phosphorylated tau protein; He, hematoxylin; GM, grey matter; WM, white matter; TDP-43, TAR DNA-binding protein 43; PLP, proteolipid protein. NIIs, intranuclear inclusion; NCIs, neuronal cytoplasmic inclusions; DNs, dystrophic neurites.
Plp 139 151 (Naive)/Cfa Emulsion, supplied by Hooke Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plp 139-151 /cfa  (Hooke Laboratories)
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Hooke Laboratories plp 139-151 /cfa
Neuropathological findings across study subjects. (A) Staining with AT8 (Tau) across superior and middle temporal samples from each study subject (left panel). (B) Higher magnification AT8 staining (right panel) from representative grey and white matter regions, indicated by squares in the left panel. (C) Immunohistochemical staining with TDP-43 across the preparations from all study subjects (left panel). (D) Corresponding magnification of grey and white matter regions (right panel), indicated by squares in the left panel. (E) Additional magnification and morphological features of TDP-43 aggregates in GM regions from FTLD subjects. Additional whole-section and high magnification images of grey and white matter stained for β-amyloid (F) and <t>proteolipid</t> protein <t>(PLP)</t> (G) are presented for each frontotemporal lobar dementia case. FTLD, frontotemporal lobar dementia; H-AD, high likelihood Alzheimer’s disease; AT8, phosphorylated tau protein; He, hematoxylin; GM, grey matter; WM, white matter; TDP-43, TAR DNA-binding protein 43; PLP, proteolipid protein. NIIs, intranuclear inclusion; NCIs, neuronal cytoplasmic inclusions; DNs, dystrophic neurites.
Plp 139 151 /Cfa, supplied by Hooke Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Neuropathological findings across study subjects. (A) Staining with AT8 (Tau) across superior and middle temporal samples from each study subject (left panel). (B) Higher magnification AT8 staining (right panel) from representative grey and white matter regions, indicated by squares in the left panel. (C) Immunohistochemical staining with TDP-43 across the preparations from all study subjects (left panel). (D) Corresponding magnification of grey and white matter regions (right panel), indicated by squares in the left panel. (E) Additional magnification and morphological features of TDP-43 aggregates in GM regions from FTLD subjects. Additional whole-section and high magnification images of grey and white matter stained for β-amyloid (F) and proteolipid protein (PLP) (G) are presented for each frontotemporal lobar dementia case. FTLD, frontotemporal lobar dementia; H-AD, high likelihood Alzheimer’s disease; AT8, phosphorylated tau protein; He, hematoxylin; GM, grey matter; WM, white matter; TDP-43, TAR DNA-binding protein 43; PLP, proteolipid protein. NIIs, intranuclear inclusion; NCIs, neuronal cytoplasmic inclusions; DNs, dystrophic neurites.

Journal: Frontiers in Neuroscience

Article Title: Fourier transform infrared spectroscopy detects distinct TAR DNA-binding protein 43 signatures in frontotemporal lobar degeneration

doi: 10.3389/fnins.2025.1649433

Figure Lengend Snippet: Neuropathological findings across study subjects. (A) Staining with AT8 (Tau) across superior and middle temporal samples from each study subject (left panel). (B) Higher magnification AT8 staining (right panel) from representative grey and white matter regions, indicated by squares in the left panel. (C) Immunohistochemical staining with TDP-43 across the preparations from all study subjects (left panel). (D) Corresponding magnification of grey and white matter regions (right panel), indicated by squares in the left panel. (E) Additional magnification and morphological features of TDP-43 aggregates in GM regions from FTLD subjects. Additional whole-section and high magnification images of grey and white matter stained for β-amyloid (F) and proteolipid protein (PLP) (G) are presented for each frontotemporal lobar dementia case. FTLD, frontotemporal lobar dementia; H-AD, high likelihood Alzheimer’s disease; AT8, phosphorylated tau protein; He, hematoxylin; GM, grey matter; WM, white matter; TDP-43, TAR DNA-binding protein 43; PLP, proteolipid protein. NIIs, intranuclear inclusion; NCIs, neuronal cytoplasmic inclusions; DNs, dystrophic neurites.

Article Snippet: Myelin proteolipid protein (PLP) staining (Serotec, Oxford, United Kingdom, 1:500) was performed by standard DAB staining procedures as previously described ( ).

Techniques: Staining, Immunohistochemical staining, Binding Assay